|
KMID : 0665220110240040803
|
|
Korean Journal of Food and Nutrition
2011 Volume.24 No. 4 p.803 ~ p.807
|
|
|
Effect of Oxygen Concentration, Physical Trauma on Proliferation of Umbilical Cord Blood-derived Mesenchymal Stem Cells
|
|
Park Ran-Sook
|
|
|
Abstract
|
|
|
|
Human umbilical Mesenchymal Stem Cell(uMSC) has been known as one of major component to regenerate connective tissues such as bone, cartilage, fat and others. The effect of low(5%), normotensive(20%) oxygen and freezing-thawing damage on proliferation of uMSC were investigated. low oxygen concentration culture of uMSC resulted in enhanced proliferation significantly(p<0.05) than 20% of oxygen culture. After the freezing-thawing injury to uMSC, 5% oxygen culture showed marked proliferation of uMSC than that of 20% oxygen(p<0.05) in the 5th passage of uMSC. Expression of antioxidant enzymes such as superoxide anion 1 and glutathione peroxidase 1 appeared marked in 20% oxygen cultured uMSC, which suggest oxidative stress could induce less proliferation of uMSC. Above findings would suggest prolferation of uMSC in 5% of oxygen will give more yields.
|
|
|
KEYWORD
|
|
|
MSC, low oxygen(hypoxia), freezing-thawing, oxidative stress
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|